منابع مشابه
Flap endonuclease of bacteriophage T7
Gene 6 protein of bacteriophage T7 has 5'-3'-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than the exonuclease activity. Unlike the human homolog of gene 6 protein, the flap endonuclease activity of gene 6 protein is dependent on the length of the 5'-flap. This dependenc...
متن کاملBacteriophage T7 endonuclease. I. Properties of the enzyme purified from T7 phage-infected Escherichia coli B.
An endonuclease activity present in extracts from Escherichia coli B infected with T7f phage has been purified 2500fold. This enzyme, T7 endonuclease I, is the product of gene 3, and its activity is maximal 7.5 min after infection at 37”. The activity is at least 100 times greater with single stranded DNA as substrate than with duplex DNA. The limit product obtained by digestion of single stran...
متن کاملRefactoring bacteriophage T7
Natural biological systems are selected by evolution to continue to exist and evolve. Evolution likely gives rise to complicated systems that are difficult to understand and manipulate. Here, we redesign the genome of a natural biological system, bacteriophage T7, in order to specify an engineered surrogate that, if viable, would be easier to study and extend. Our initial design goals were to p...
متن کاملRescue of bacteriophage T7 DNA polymerase of low processivity by suppressor mutations affecting gene 3 endonuclease.
The DNA polymerase encoded by gene 5 (gp5) of bacteriophage T7 has low processivity, dissociating after the incorporation of a few nucleotides. Upon binding to its processivity factor, Escherichia coli thioredoxin (Trx), the processivity is increased to approximately 800 nucleotides per binding event. Several interactions between gp5/Trx and DNA are required for processive DNA synthesis. A basi...
متن کاملSuperinfection exclusion by bacteriophage T7.
Only two of the early genes of bacteriophage T7 were found to play a significant role in exclusion of superinfecting bacteriophage T3 particles; genes 0.3 and 1. Protein synthesis by the preinfecting phage particle was not required for efficient exclusion. These findings are discussed with regard to the known functions of these genes during T7 development.
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ژورنال
عنوان ژورنال: Bacteriophage
سال: 2014
ISSN: 2159-7081
DOI: 10.4161/bact.28507